cardiac troponin t (Developmental Studies Hybridoma Bank)
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Cardiac Troponin T, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jlt12/anti-Troponin+T/pmc13097457-141-23-27
Average 96 stars, based on 269 article reviews
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Western Blot:Article Title: Desensitizing mouse cardiac troponin C to calcium converts slow muscle towards a fast muscle phenotype Article Snippet: .. Several prominent and several minor bands were consistently observed in the samples probed on immunoblots with the anti‐fast‐type TnT antibody, Article Title: Desensitizing mouse cardiac troponin C to calcium converts slow muscle towards a fast muscle phenotype Article Snippet: .. Several prominent and several minor bands were consistently observed in the samples probed on immunoblots with the anti-fast-type TnT antibody, Blocking Assay:Article Title: Metastatic cancers promote cachexia through altered zinc homeostasis in skeletal muscle Article Snippet: Protein concentration was determined by Pierce BCA protein assay kit (Thermo scientific), and proteins were resolved by SDS-PAGE and transferred onto Polyvinylidene difluoride membranes. .. After blocking with 5% w/v non-fat milk in TBS-T (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween 20) for 1 hour at room temperature, the membranes were incubated with the following antibodies: rabbit polyclonal antibodies against ZIP14 (1:1000) (Millipore, 06-1022, NRG1830910), p-c-Jun (S63) (1:1000) (Cell signaling, #9261) and Cleaved-caspase 3 (1:1000) (Cell signaling, #9661); rabbit monoclonal antibodies against p-p65 (S536) (#3033), p65 (#8242), c-Jun (#9165), pSmad2 (S465/467) (#3108), Smad2 (#5339 purchased from Cell signaling with a dilution of 1:1000); mouse monoclonal antibodies against fast MyHC (1:5000) (Sigma, clone MY-32), skeletal actin (1:5000) (Sigma, A2172), tubulin (1:5000) (Sigma, T6074), troponin T (1:1000) (DSHB, Incubation:Article Title: Metastatic cancers promote cachexia through altered zinc homeostasis in skeletal muscle Article Snippet: Protein concentration was determined by Pierce BCA protein assay kit (Thermo scientific), and proteins were resolved by SDS-PAGE and transferred onto Polyvinylidene difluoride membranes. .. After blocking with 5% w/v non-fat milk in TBS-T (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween 20) for 1 hour at room temperature, the membranes were incubated with the following antibodies: rabbit polyclonal antibodies against ZIP14 (1:1000) (Millipore, 06-1022, NRG1830910), p-c-Jun (S63) (1:1000) (Cell signaling, #9261) and Cleaved-caspase 3 (1:1000) (Cell signaling, #9661); rabbit monoclonal antibodies against p-p65 (S536) (#3033), p65 (#8242), c-Jun (#9165), pSmad2 (S465/467) (#3108), Smad2 (#5339 purchased from Cell signaling with a dilution of 1:1000); mouse monoclonal antibodies against fast MyHC (1:5000) (Sigma, clone MY-32), skeletal actin (1:5000) (Sigma, A2172), tubulin (1:5000) (Sigma, T6074), troponin T (1:1000) (DSHB, Bioprocessing:Article Title: Metastatic cancers promote cachexia through altered zinc homeostasis in skeletal muscle Article Snippet: Protein concentration was determined by Pierce BCA protein assay kit (Thermo scientific), and proteins were resolved by SDS-PAGE and transferred onto Polyvinylidene difluoride membranes. .. After blocking with 5% w/v non-fat milk in TBS-T (20 mM of Tris-HCl pH 7.4, 500 mM of NaCl and 0.1% of Tween 20) for 1 hour at room temperature, the membranes were incubated with the following antibodies: rabbit polyclonal antibodies against ZIP14 (1:1000) (Millipore, 06-1022, NRG1830910), p-c-Jun (S63) (1:1000) (Cell signaling, #9261) and Cleaved-caspase 3 (1:1000) (Cell signaling, #9661); rabbit monoclonal antibodies against p-p65 (S536) (#3033), p65 (#8242), c-Jun (#9165), pSmad2 (S465/467) (#3108), Smad2 (#5339 purchased from Cell signaling with a dilution of 1:1000); mouse monoclonal antibodies against fast MyHC (1:5000) (Sigma, clone MY-32), skeletal actin (1:5000) (Sigma, A2172), tubulin (1:5000) (Sigma, T6074), troponin T (1:1000) (DSHB, |
